Process Chain of BioPrinting with Brinter™

1. IMAGE ACQUISITION

Image the organ or tissue to be bioprinted (e.g., with magnetic resonance imaging (MRI), computed tomography (CT), X-ray, or ultrasound)
Obtain ready-to-print sample models of organs and tissues from the National Institutes of Health (NIH) repository: https://3dprint.nih.gov/

Segment image raw data using image-processing software.
Create a 3D CAD surface model in .STL format from image segmentation. This surface model approximates the outer shape of the construct using triangle mesh.
The surface model is then filled with repeating unit cells to generate the complete construct with internal infrastructure.
Brinter™ software generates a toolpath plan as a g-code file from the 3D model. This provides the motion path for the bioprinter to deposit bioink at the proper time and location.

Segment image raw data using image-processing software.
Create a 3D CAD surface model in .STL format from image segmentation. This surface model approximates the outer shape of the construct using triangle mesh.
The surface model is then filled with repeating unit cells to generate the complete construct with internal infrastructure.
Brinter™ software generates a toolpath plan as a g-code file from the 3D model. This provides the motion path for the bioprinter to deposit bioink at the proper time and location.

A cell-laden hydrogel solution is prepared by mixing cells with the chosen hydrogel solution.
Prepared bioink is loaded into the Brinter™ bioprinter.
Surface model (g-code file) is read into Duet3D software controlling the printing process.
3D bioprinter builds the tissue model layer-by-layer.
Cell-laden construct can be chemically or physically cross-linked after printing for structural integrity.