Process Chain of BioPrinting with Brinter™

1. IMAGE ACQUISITION

  • Image the organ or tissue to be bioprinted (e.g., with magnetic resonance imaging (MRI), computed tomography (CT), X-ray, or ultrasound)
  • Obtain ready-to-print sample models of organs and tissues from the National Institutes of Health (NIH) repository: https://3dprint.nih.gov/
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2. IMAGE PROCESSING

  • Segment image raw data using image-processing software.
  • Create a 3D CAD surface model in .STL format from image segmentation. This surface model approximates the outer shape of the construct using triangle mesh.
  • The surface model is then filled with repeating unit cells to generate the complete construct with internal infrastructure.
  • Brinter™ software generates a toolpath plan as a g-code file from the 3D model. This provides the motion path for the bioprinter to deposit bioink at the proper time and location.

2. IMAGE PROCESSING

  • Segment image raw data using image-processing software.
  • Create a 3D CAD surface model in .STL format from image segmentation. This surface model approximates the outer shape of the construct using triangle mesh.
  • The surface model is then filled with repeating unit cells to generate the complete construct with internal infrastructure.
  • Brinter™ software generates a toolpath plan as a g-code file from the 3D model. This provides the motion path for the bioprinter to deposit bioink at the proper time and location.
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3. 3D BIOPRINTING

  • A cell-laden hydrogel solution is prepared by mixing cells with the chosen hydrogel solution.
  • Prepared bioink is loaded into the Brinter™ bioprinter.
  • Surface model (g-code file) is read into Duet3D software controlling the printing process.
  • 3D bioprinter builds the tissue model layer-by-layer.
  • Cell-laden construct can be chemically or physically cross-linked after printing for structural integrity.

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BRINTER™ 1

Revolutionary Modular Bioprinter

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